Liquid nitrogen free and cryogen free controlled rate freezer for the cryopreservation of a wide
range of material including: embryos, stem cells, mammalian cells, spermatozoa, antibodies,
tissue sections and rodent organs. The EF600M brings accuracy, precision and
reproducibility to biological cryopreservation.
Unlike conventional liquid Nitrogen based controlled rate cooling equipment used in IVF laboratories, the EF600M
poses no contamination risk and can be used in cleanrooms and barrier facilities. The EF600M fits neatly and quietly
on a bench-top and its performance in terms of cell viability after freezing is comparable or better than standard
liquid nitrogen freezers. As alcohol is not used, there is also no potential fire risk. The EF600M will cool down to –
100ºC with straws.
The cooling rate of the EF600M is precisely controlled, ensuring accuracy and reproducibility throughout the freezing
profile especially for the important nucleation/seeding phase. This ensures optimal recovery of cells upon thawing.
Operation is simple and can be carried out with or without a PC; data can be logged via PC software and cooling
profiles are directly displayed. Different cooling profiles are available from a drop down menu and customised profiles
can be written. Published trials have demonstrated successful freezing and recovery of embryos, sperm and
embryonic stem cells.
Main Applications
The EF600M is highly versatile and can be used for the cryopreservation of a wide range of samples in cryovials,
straws, bags, microplates and Matrix-96-well block plates including:
 Human and veterinary IVF
Transgenic embryos
Stem cells
Clinical and research samples, e.g. lymphocytes and tissue cells lines in conventional cryovials
Various mammalian cells including cardiomyocytes, adipose, liver and muscle
Cord blood derived stem cells in typical cryocyte bags
Adherent cells and stem cells in microplates plates
Cell suspensions in numbered/barcoded arrays for archiving and storage
Robotic integration – the EF600M has also successfully been integrated into robotic systems, supporting
biorepository and biobank applications
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Key Features
Accurate and reproducible control of cooling rates and sample temperatures
Easy to use and samples can be nucleated/seeded in-situ
Linear and non-linear cooling profiles
Low running costs: estimated at 1% of liquid nitrogen controlled rate freezing
Temperature remains at -100ºC at the end of cycle for straw applications until freezer is switched off
Uninterruptible Power Supply (UPS): complete cycle run if power fails (supplied as an optional accessory)
CE marked
Servicing and calibration available
3 year warranty
Data Sheet
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